Analysis of Yeast Insert Sequences
- Open the NSLC Server Folder on the Desktop.
- Open the BIO 2960 Folder.
- Open the Yeast Sequence folder, your lab instructor will tell you what sequence to select.
- Double click on your sequence. This will open FinchTV, the DNA sequence viewing program.
- Under the Edit Menu select Select BLAST sequence-Nucleotide BLASTn or hold-down shift, apple, and "B". This will open the default internet browser, take you directly to the NCBI website (databases), and copy/paste your DNA sequence into the search window.
- In the Choose Search Set: Database, click on the button corresponding to "Others (nr etc)".
- Click on the button. Wait for the results of your search. BE PATIENT, THIS MAY TAKE A FEW MINUTES.
- Once you get the results of your BLAST search, look for a specific gene name from yeast (Saccharomyces cerevisiae). A gene name usually consists of three letters followed by a number. Do not use an ORF number, a clone number or a chromosome number. You may need to click on the accession numbers on the left to find a gene name (Roman numeral).
- Once you have a gene name, click here. To get information about the specific gene, just type the gene name into the quick search box and click submit.
- Complete the questions in the lab report about the gene and gene product.