16s rRNA Gene Sequence Analysis

A. Retrieving and viewing your DNA sequence
  1. Open the NSLC Data Folder on the Desktop.
  2. Open the BIO 2960 Folder.
  3. Open the folder for your lab section and identify your sequence.
  4. Double click on your sequence. This will open FinchTV, the DNA sequence viewing program, and your sequence in the GRAPHIC mode. To view your sequence in the TEXT mode, hold-down the apple button and press "i".
  5. Drag your sequence onto the FinchTV application icon located in the dock, if FinchTV doesn't automatically open after you doubled clicked on your sequence.

B. Searching the NCBI database for sequences that are homologous with your sequence with FinchTV