An introduction to laboratory techniques for experimental manipulation of DNA (and RNA) molecules, including construction, isolation, and analysis of plasmids, RNA, PCR products and sequencing. A molecular cloning experiment, RNA isolation, RT-PCR, Southern analysis and plant transformation are performed as class projects. Fulfills the laboratory requirement of the biology major. Prerequisites: Biol 297A, 3051, Chem 152, and permission of instructor. One hour of lecture and eight hours of laboratory each week. Credit 4 units.

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