This course focuses on methods for the engineering, manipulation and analysis of proteins. In the course, we will begin by purifying a thermostable DNA polymerase and then use it to clone, by polymerase chain reaction (PCR)-amplification the coding regions for a number of genes encoding proteins of current research interest. These proteins will be engineered for overexpression in E. coli and some will be fused to yellow fluorescent protein for visualization in transformed plant cells using fluorescence microscopy. Along the way, we will learn about thermal stabilities of proteins, ion-exchange and/or affinity chromatography, measurement of protein concentrations, assessment of protein purity by SDS polyacrylamide gel electrophoresis, analysis of enzyme function, protein identification by mass spectrometry and an introduction to structural analysis through X-ray crystallography. Prerequisites: Chem 252 and Bio 2960 (or equivalent). Enrollment is limited and requires the permission of Dr. Pikaard. Credits: 3Spring semester, Monday 3:00-4:00; Wednesday 12:00-4:00; Friday 12:00-4:00

Location: Monday lectures will be held in McDonnell Hall 412. Laboratory sessions will be held primarily in Rebstock Hall 126. Some lab sessions will at the Donald Danforth Plant Science Center (DDPSC), depending on the topic.