Course Materials

Course coordinators:

  1. Prof. Craig Pikaard, Biology Department, pikaard@biology.wustl.edu; phone: 314-935-7569; Office: Monsanto Hall Room 505
  2. Dr. Joseph Jez, DDPSC and Adjunct Assistant Professor of Biology at Wash. U., e-mail: jjez@danforthcenter.org

Instructors:

Teaching assistants:

Lecture
 PowerPoint Slides Readings
1
 Basic principles of protein biochemistry

2
 Gateway recombinational cloning Earley et al.
3
 Protein Expression and Purification Protein Purification Handbook
4
 Sequencing etc.
5
 Danforth Center
6
 TC and tranformation
7
 Composite plant
8
 Enzymes, Assays, Kinetics
9
 Protein Crystallography Joseph M. Jez et al.

Labs
 Protocols/Notes
1&2
  1. Protocols
  2. General lab notes
3
  1. Protocols
4
  1. Protocols
5
  1. PPT-Slides
  2. Gel purification of PCR products and cloning into pENTR
  3. QIAquick Gel Extraction Kit
  4. Preparative and analytical purification of DNA from agarose
6
7
  1. Protocols
  2. Transfer of sequences cloned in Gateway pENTR vector to Gateway destination vectors: LR clonase-mediated recombination reactions
8
  1. Protocols
  2. Digest Chart PDF file and EXCEL file
9
  1. Protocols
10
  1. Protocols-SDS-PAGE
  2. Protein Crystallography
11
  1. Protocols
  2. Trypsin digestion lab
12
  1. Class Schedule
  2. Electroporation into Agrobacterium
  3. Lab Images-Large File
13
  1. Protocols
14
  1. Protocols
Natural Sciences Learning Center
Washington University - Biology
Copyright © 2008